Direct numerical simulation of a fully developed turbulent square duct flow up to Re-tau=1200

Various fundamental studies based on a turbulent duct flow have gained popularity including heat transfer, magnetohydrodynamics as well as particle-laden transportation. An accurate prediction on the turbulent flow field is critical for these researches. However, the database of the mean flow and turbulence statistics is fairly insufficient due to the enormous cost of numerical simulation at high Reynolds number. This paper aims at providing available information by conducting several Direct Numerical Simulations (DNS) on turbulent duct flows at Re-tau = 300, 600, 900 and 1200. A quantitative comparison between current and previous DNS results was performed where a good agreement was achieved at Re-tau = 300. However, further comparisons of the present results with the previous DNS results at Re-tau = 600 obtained with much coarser meshes revealed some discrepancies which can be explained by the insufficient mesh resolution. At last, the mean flow and turbulent statistics at higher Re-tau was presented and the effect of Re-tau on the mean flow and flow dynamics was discussed.Peer ReviewedPostprint (author's final draft

Particulate immersed boundary method for complex fluid-particle interaction problems with heat transfer

In our recent work (Zhang et al., 2015), a Particulate Immersed Boundary Method (PIBM) for simulating fluid-particle multiphase flow was proposed and assessed in both two- and three-dimensional applications. In this study, the PIBM was extended to solve thermal interaction problems between spherical particles and fluid. The Lattice Boltzmann Method (LBM) was adopted to solve the fluid flow and temperature fields, the PIBM was responsible for the no-slip velocity and temperature boundary conditions at the particle surface, and the kinematics and trajectory of the solid particles were evaluated by the Discrete Element Method (DEM). Four case studies were implemented to demonstrate the capability of the current coupling scheme. Firstly, numerical simulation of natural convection in a two-dimensional square cavity with an isothermal concentric annulus was carried out for verification purpose. The current results were found to have good agreement with previous references. Then, sedimentation of two-and three-dimensional isothermal particles in fluid was numerically studied, respectively. The instantaneous temperature distribution in the cavity was captured. The effect of the thermal buoyancy on particle behaviors was discussed. Finally, sedimentation of three-dimensional thermosensitive particles in fluid was numerically investigated. Our results revealed that the LBM-PIBM-DEM is a promising scheme for the solution of complex fluid-particle interaction problems with heat transfer.Peer ReviewedPostprint (author's final draft

Preparation of Equine Immunoglobulin F(ab′) 2 against Smallpox and Evaluation of its Immunoprotective Effect

Smallpox, a severe infectious disease caused by the smallpox virus, causes a death rate as high as 30% within 15-20 days after infection. Therefore, development of anti-Smallpox product as a strategic reserve is urgently needed. We prepared and tested pepsin-digested F(ab′) 2 fragments of serum IgG from horses. Transmission electron microscopy indicated that the purified virus showed morphology consistent with VVTT. The titer was above 1.0 × 10 7 PFU/mL. The purity of the antigen exceeded 90%, according to HPLC. After purification and cleavage, the yield of the purified product F(ab′) 2 was approximately 1.3%, its purity exceeded 90%, and the neutralizing antibody titer exceeded 1:3200. F(ab′) 2 fragments had good preventive and therapeutic effects in mice at antibody doses of 5.2 mg/mL and 2.6 mg/mL. The viral loads of the drug-treated mice were suppressed to varying degrees, and the higher dose groups (5.2 and 2.6 mg/mL) showed a 2-3 fold lower viral load than that in the control group. A process for producing equine immunoglobulin F(ab′) 2 against VVTT was established. The prepared horse anti-smallpox immunoglobulin product had good neutralizing antibody effects on VVTT. The highly purified preparation may serve as a potential candidate for smallpox treatment

Purified Immunoglobulin F(ab′) 2 Protects Mice and Rhesus Monkeys against Lethal Ricin Intoxication

Ricin is a highly toxic ribosome-inactivating lectin derived from castor beans. To date, no antidote is available to treat ricin-poisoned patients, and the development of a safe and effective antidote is urgently needed. First, ricin was prepared and used to construct a mouse model and a rhesus monkey model of ricin intoxication. Second, pepsin-digested F(ab′) 2 fragments of serum IgG from horses injected with Freund’s-adjuvanted purified ricin were prepared. Third, the protective efficacy was evaluated in mouse and rhesus monkey models of lethal ricin intoxication. The purity quotient of the prepared ricin and F(ab′) 2 fragments exceeded 90% and 85% in the mouse and monkey models, respectively. The LD 50 of ricin in mice and rhesus monkeys was 2.7 and 9 μg/kg, respectively. A quantity of 6.25 and 1.85 mg/kg F(ab′) 2 was sufficient to treat lethal ricin intoxication in the mice and rhesus monkeys, respectively. Finally, the effect of this therapeutic antibody on peripheral blood immune cells was examined by analysis of peripheral blood immune cells through single cell sequencing. The underlying mechanism was found to involve restraining neutrophil activation, proliferation, and differentiation. Purified F(ab′) 2 fragments administered with needle-free devices fully protect mice and rhesus monkeys against lethal doses of ricin intoxication

PIBM: Particulate immersed boundary method for fluid-particle interaction problems

It is well known that the number of particles should be scaled up to enable industrial scale simulation. The calculations are more computationally intensive when the motion of the surrounding fluid is considered. Besides the advances in computer hardware and numerical algorithms, the coupling scheme also plays an important role on the computational efficiency. In this study, a particulate immersed boundary method (PIBM) for simulating the fluid–particle multiphase flow was presented and assessed in both two- and three-dimensional applications. The idea behind PIBM derives from the conventional momentum exchange-based Immersed Boundary Method (IBM) by treating each Lagrangian point as a solid particle. This treatment enables Lattice Boltzmann Method (LBM) to be coupled with fine particles residing within a particular grid cell. Compared with the conventional IBM, dozens of times speedup in two-dimensional simulation and hundreds of times in three-dimensional simulation can be expected under the same particle and mesh number. Numerical simulations of particle sedimentation in Newtonian flows were conducted based on a combined LBM–PIBM–Discrete Element Method (DEM) scheme, showing that the PIBM can capture the feature of particulate flows in fluid and is indeed a promising scheme for the solution of the fluid–particle interaction problems

Discrete element simulation of particle motion in ball mills based on similarity

Discrete element (DE) simulation of a ball mill with a large number of particles is challenging when each particle is considered. Similarity principle could be adopted to reduce the number of particles in a simulation whilst still maintaining the accurate flow behaviour of particles. This paper presents a scaling relationship between particle gravitational acceleration, mill diameter and mill rotational speed. A series of scaled simulations of particle motion with different mill diameters are carried out. Consistent motion of a single particle and multiple particles in ball mills with different diameters and rotational speeds verifies the proposed relationship, which could be an effective approach to reduce the size of simulations for ball mills

Discrete element modeling of the microbond test of fiber reinforced composite

Discrete element method (DEM) was used to simulate the dynamic process of microbond test of fiber reinforced composites, in which the fiber and matrix were modeled as elastic and elastic-plastic materials, respectively. The interface between fiber and matrix was represented by a bilinear contact softening model. Plastic deformation and progressive cracking of the matrix were observed in the simulation with comparable similarity to the existing experimental results. The initiation and propagation of interfacial debonding were also captured by the DEM simulations, whereas it is very difficult to achieve this by other numerical methods. Vertical and inclined vises with two different vise angles were tested in the simulations. It was found that both vise geometry and vise angle had significant effect on the damages of the material, and the inclined vise was sensitive to the vise angle in terms of large variation of cutting force. The developed DEM model can also be applied to predict material damages in other more complicated fiber reinforce composite system. (C) 2010 Elsevier B.V. All rights reserved

Multi-scale modelling of damage progression in FRC laminates - Applications of DEM

As a natural progress of the research in the area of modeling damage at microscopic scales, a discrete element method (DEM) has been proposed to simulate the damage progression in FRC laminates. DEM has been used to study the interfacial debonding, transverse cracking, delamination, and transverse cracking and delamination in FRC laminates. The purpose of this research is not only to validate the application of DEM in terms of its advantages in the simulation of damage progression and the prediction of cracking density and stiffness reduction, but also to highlight the potential of DEM in the future research application for composite damage mechanism, composite material design and optimization